Skop Lab resources

Onboarding
Lab Expectations
Lab Meetings
​Lab Jobs, etc
Code of Conduct

Undergraduate Research Rubric
Undergraduate Final Presentation Rubric

Biosafety Guide for new students

Henrietta Lacks

Microskops

Our lab houses several microscopes including a brightfield Revolve Echo scope, a spinning disk confocal, Tomocube Holotomography Scope, and a phase scope for tissue culture use. All are available to use.  Please email Ahna ([email protected]) and Liz Torr ([email protected]) for calendar use

• Revolve ECHO Scope
• Tomocube Holotomography Scope: Label free imaging using Refractive Index (RI) which can be combined with GFPs/Dyes/etc: email [email protected]
• Skop Lab Spinning Disk: email [email protected]
• ​Tissue Culture Phase Contrast

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Skop Lab Tissue Culture protocols

Tissue Culture
Skop Lab Fixation Protocol for tissue culture cells (adapted from Salmon Lab)

Midbody (MB) isolation 
Ahna's MB isolation from (Skop, 2004, Science)

MBR isolation
Skop lab MBR isolation and fixation for imaging (Park, Patel, 2023, Cell Star Protocols)
Skop Lab MBR isolation using 1.5% PEG (Park, 2023, Cell Star Protocols)
Skop Lab MBR isolation using gold nanoparticles (Park, 2023, Cell Star Protocols)
Skop Lab MBR isolation using ultra centrifuge (Park, 2023, Dev Cell, Patel et al, 2024 MBoC)
Skop Lab MBR counting Macro for Image J (Patel, et al, 2024 MBoC) (GITHUB)

RNA imaging
Tissue Culture and MB/MBR RNAscope protocol

MBR translation
Skop Lab MB and MBR puromycin fixation to detect translation (Park, 2023, Dev Cell: Patel, et al 2024, MBoC)
Skop Lab MB and MBR ClickIT fixation to detect active translation (Park, 2023, Dev Cell)

C. elegans Protocols